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WebThe cell cycle was determined using a Propidium Iodide Kit (Invitrogen, Carlsbad, CA, USA). Briefly, after washing three times with PBS, the cells were fixed with 4 mL of 75% ethanol for 4 h at 4°C. The cells were washed twice with PBS and incubated for 30 minutes at 37°C with 100 μL of 200 μg/mL DNase-free RNaseA. WebTEXT US @ (858) 365-9492. Carlsbad and surrounding areas Car Detailing. San Diego’s best choice for mobile Auto Detailing and Car Wash services directly at your home or office. …
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