Phis2-p53

Author: mddj

August undefined, 2024

Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 … http://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825

p73 or p53 Directly Regulates Human p53 Transcription to …

WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: … WebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous how many baptists in uk

知无不“研” 一文读懂酵母单/双杂交点对点验证 - 知乎

http://www.coolaber.com/usr/2160/File/F/F4671.pdf WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb1）准备质粒：构建pHis2-bait质粒→提取质粒； 2）制备Y187感受态细胞：YPDA平板活化→YPDA液体扩繁→制备酵母感受态细胞；或者直接购买Y187感受态细胞（CC306）； … high plowback ratio

p53-His,pLacZ,pHis，pHisi-1_MZ菌株_宁波明舟生物科技有限公 …

Addgene: Vector Database - pHIS-2

Webbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees … Webb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM … how many baptists in ukraineWebb上海北诺生物科技有限公司所提供的酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，质量可靠、规格齐全,上海北诺生物科技有限公司不仅具有专业的技术水平,更有良好的售后服务和优 … high plow fat snacks

"Webb1 mars 2024 · The pHIS2-p53/pGADT7-Rec2-p53 and pHIS2-p53/pGADT7-Rec2-PtrHAT22 were used as the positive and negative control, respectively. 10 −1, 10 −2, and 10 −3 represent solution dilution ratio of transformed Y187 yeast cells. 4. Discussion. " - Phis2-p53

Phis2-p53

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Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … WebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community …

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http://www.engineeredprocess.com/images/Honeywell_IFM_P531.P532_IOM.pdf WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article …

Webb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基 Webb4 okt. 2013 · The interactions of pHIS2-p53 (three tandem copies of the cis-acting DNA consensus sequence in pHIS2, which is recognized by p53) with the tested TFs were used as negative controls. Two MYC ( ThMYC4 and ThMYC6 GenBank number: JN166788 and JN166790) were identified to bind to the E-box motif, of which ThMYC6 that bound with …

Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His … Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by …

WebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without …

http://www.zoonbio.com/molecular/yeast-one-hybrid-procedure.html high plt blood countWebb2、酵母双杂交酵母双杂交及系统是一种鉴定和检测蛋白质相互作用的研究方法，因其具有灵敏性高、功能强大、适用范围广等特点，现已被应用于多个研究领域。 ①核蛋白酵母 … how many baptists in the worldWebb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 … how many baptists are there worldwideWebb5 apr. 2024 · The Y187 strain co-expressing pGADT7-LkARF7 and pHIS2-LkBBM1 and the strain co-expressing pGADT7-LkARF19 and pHIS2-LkBBM1 grew normally on TDO medium containing 75 mM 3-AT, ... The bait vectors pGBKT7-p53 and pGBKT7-LaminC were co-transformed into AH109 with the prey vector pGADT7-LargeT as positive and negative … how many bar is 5000 psiWebbpBIND pACT-MyoD pBIND-Id pG5 luc pCMV-BD pCMV-AD pBD-p53 pBD-NF−κB pAD-SV40T pAD-TRAF pFR-luc pGBKT7 pGADT7 pCL1 pGBKT7-53 pGADT7-T pGBKT7-Lam pACT2 AD Y187 AH109 pSos pMyr pSos MAFB pHis2 pHisSi-1 p53blue pGAG424 pLaczi p53his pGAD53m pBridge Y2H Gold Yeast strain Y187 pIND how many bar chairs per m2Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast … high platform silver heelsWebbOur Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbA r) as a stringent, highly selective, and easy-to-use reporter. how many baptists in the usa