WebMay 1, 2005 · Cytokinin dehydrogenase was confirmed to be present in the apoplast of cells. Co-staining of enzyme activity for laccase, an enzyme poised to function on the cell wall in the apoplast, in those tissues suggests a possible cooperation of the enzymes in cytokinin degradation. WebMay 1, 1999 · Similar to Cytokinin dehydrogenase 5 precursor (EC 1.5.99.12) (Cytokinin oxidase 5) (CKO5) (AtCKX5) (AtCKX6) Location. Chromosome 1: 32,788,488-32,792,747 reverse strand. About this gene. This gene has 1 transcript (splice variant), 154 orthologues and 17 paralogues. Transcripts. Show transcript table Hide transcript table.
Cytokinin dehydrogenase: a genetic target for yield
To identify an omnipresent FAD-dependent protein, that could be related to the cytokinin binding domain we performed a pBlast search. By application of pBlast search, we have found that on the sequence level the closest to CKX protein in Arabidopsis thalianais the FAD-linked D-2-hydroxyglutarate … See more To track the evolutionary origin of the CKXs, we have searched for the homologous sequences from the Uniprot, Pfam, and NCBI databases using the homology-based … See more Because our phylogenetic tree analysis could not answer the question regarding the origin of the cytokinin binding domain, we have analysed structural models of the CK-binding domains. … See more Domain architecture plays a key role in understanding the functionality of proteins along with their evolutionary history. To evaluate the gain … See more To understand the evolutionary history of the C-terminal cytokinin binding domain proteins the phylogenetic trees were inferred with the … See more Webendogenous cytokinin concentrations are nanomolar [15]. Enzyme based amperometric biosensors provide a simple, fast, selective, convenient and low cost analytical technique. Cytokinin dehydrogenase (CKX; EC 1.5.99.12) degrades cytokinins by oxidative cleavage of N6- side chain using FAD as the electron-donating cofactor [16–19]. high swings free
Gene: OsCKX5 (Os01g0775400) - Summary - Oryza_sativa
WebAug 21, 2007 · Transgenic tobacco plants overexpressing single Arabidopsis thaliana cytokinin dehydrogenase (CKX, EC 1.5.99.12) genes AtCKX1, AtCKX2, AtCKX3, AtCKX4, AtCKX5, AtCKX6, and AtCKX7 under the control of a constitutive 35S promoter were tested for CKX-enzymatic activity with varying pH, electron acceptors, and substrates. This … WebSince 1980s, it has already been established that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; however, the potential … WebDec 7, 2015 · Here, we show that the Lotus japonicus Ckx3 cytokinin oxidase/dehydrogenase gene is induced by Nod factor during the early phase of nodule initiation. ... Quantification was obtained using a multiple reaction-monitoring mode of selected precursor ions and the appropriate product ion. GenBank accession numbers: … how many days to go to moon